（备注：非常规分子量：400、600。精确聚合度的小分子定制。）

结构式：

基本信息：

中文名称：二苯并环辛烯-聚乙二醇-叶酸 (DBCO-PEG-FA)

别名：DBCO-PEG-FA

英文名称：DBCO-PEG-FA，DBCO-PEG- Folic acid

CAS：-

分子量：400、600、1K、2K、3.4K、4K、5K、10K

纯度：90%以上

应用简介：

DBCO PEG Folic acid, also called DBCO PEG folate, is one of Ponsure’ folic acid PEG derivatives that can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG folate derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without

the need for toxic Cu catalysts.

Folic acid, is also known as vitamin M, vitamin B9 or pteroyl-L-glutamic acid.

Folic acid is an essential bioactive molecule for numerous biological functions. It participates in thesynthesis, repairing and methylation of DNA as well as to act as a cofactor in many biological reactions. DBCO functional folic acid can be easily attached to azide functionalized molecules, particles or solid surfaces.

Copper-free Click Reaction Procedures:

Prepare the azide-containing sample in reaction buffer.

Add DBCO conjugate to azide containing sample. Recommendation: Add 1 mole equivalent of limiting reagent to 1.5-3.0 mole equivalents of highest abundance reagent.

Incubate the reaction at room temperature for 2-4 hours or 2-12 hours at 4 degree celcius.

The reaction is now ready for purification.

物理性质：

状态：淡黄色到黄色固体，或者半固体，取决于分子量。

溶解性：溶于DMSO。

保持条件：-20℃长期保存，避光，干燥。

注意事项：取用一定要干燥，避免频繁的溶解和冻干。

反应性：DBCO于含有N3基团的反应。

技术指标：

纯度（PEG）：90%以上

取代率（DBCO）：90%以上

取代率（FA）：90%以上

分散系数（PDI）：小于等于1.05

分析：COA、GPC、NMR、MALDI-TOF