（备注：非常规分子量：350、500、550、750、1k。精确聚合度的小分子定制。）

结构式：

基本信息：

中文名称：甲氧基聚乙二醇-dibenzocyclooctyne (MPEG-DBCO)

别名：MPEG-ADIBO, MPEG-DBCO

英文名称：mPEG-DBCO ; mPEG-Dibenzocycolctyne

CAS：-

分子量：350、500、550、750、1K、2K、5K、10K、20K、30K

纯度：95%以上

应用简介：

mPEGDBCO, also called methoxy PEG DBCO, is a pegylated DBCO (dibenzocyclooctyne)derivative that can react with azide group without need any metal catalyst. Thestrain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, alsotermed as the Cu-free Click reaction, is a bioorthogaonal reaction that enablesthe conjugation of two molecules in aqueous solution. PEG DBCO derivatives haveexcellent water solubility, possess fast kinetics and stability in aqueousbuffer. They are one of the best pegylation reagents to modify proteins,peptides, antibodies, biopolymers and particles via copper free Click Chemistryreaction.

Copper-freeClick reaction procedures:

Preparethe azide-containing reagents in reaction buffer.

Add PEGDBCO reagents to azide containing reagents. Add 1 mol equivalent of limitingreagent to 1.5~3.0 mol equivalents of abundance reagent.

Incubatethe reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.

Purifyconjugates either by dialysis or size-exclusion chromatograph.

物理性质：

状态：淡黄色到白色固体，或者半固体，取决于分子量。

溶解性：溶于水，乙醇，DMSO等溶剂。

保持条件：-20℃长期保存，避光，干燥。

注意事项：取用一定要干燥，避免频繁的溶解和冻干。

反应性：DBCO于含有N3基团的反应。

技术指标：

纯度（PEG）：95%以上

取代率（DBCO）：95%以上

分散系数（PDI）：小于等于1.05

分析：COA、GPC、NMR、MALDI-TOF